Total RNA was extracted from cultivated viruses with an RNeasy minikit (Qiagen, CA, USA) and then reverse-transcribed using SMARTScribe reverse transcriptase (Clontech, CA, USA) and random hexamers. Furthermore, two cases (a 1-year-old female and a 30-year-old male) had a history of close contact with known imported cases from Indonesia, according to a national epidemiological investigation. Here, we report the full-length genome sequences of MeV genotype D8 isolates obtained using Vero-hSLAM cells ( 4) from throat swab specimens of three presumed import-related cases, including a 38-year-old male who works at the Gimpo International Airport with no international travel history or clear epidemiological linkage with known domestic measles cases. Several imported and import-related measles cases occurred sequentially in South Korea in 2016. However, comparing these short sequences is not sufficiently informative for distinguishing some transmission routes, so whole-genome sequencing data will be helpful for analyzing strains ( 3). For genotyping, the WHO recommends analyzing a sequence of 450 nucleotides in the C-terminal region of the N gene (N-450) ( 2). Laboratory-based MeV surveillance has emphasized the importance of adequate genetic analysis to verify virus elimination in a region, specifically through demonstrating the absence of endemic cases. Genetic information is a key factor for classifying viruses as endemic or imported, improving our understanding of virus transmission pathways ( 1). Vaccination can prevent infection, and accelerated immunization programs using a safe and cost-effective vaccine have decreased the global rate of measles-related deaths ( ). Measles is a highly contagious disease caused by the measles virus (MeV genus Morbillivirus, family Paramyxoviridae).

Sequencher 3.1